Biotin Labeling Reagents (in stock)


A broad range of biotin labeling reagents. Amine reactive, carboxyl reactive, carbonyl reactive, water-soluble, cleavable.


Broad class of PEGylated, water-soluble reagents for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules. PEGylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution.
Amine Reactive

Broad class of PEGylated, water-soluble reagents for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules. PEGylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution.

Biotin Acid

Biotin Acid is a class of linear heterobifunctional reagents with biotin at one end and carboxylic acid at the other end. These reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecule in the presence of a coupling reagent.


Biotin-NHS ester

This is the most popular subclass of biotinylation reagent. These reagents enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules. NHS-activated biotin compounds can react efficiently with primary amino groups (-NH2) to form stable, irreversible amide bonds.


Biotin-EDA-NHS

These reagents enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules. NHS-activated biotin compounds can react efficiently with primary amino (-NH2) to form stable, irreversible amide bonds.


Biotin-PFP

Biotin-PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. It reacts with primary amino (-NH2) to form stable, irreversible amide bonds.


Biotin-EDA-PFP

Biotin-EDA-PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. It reacts with primary amino (-NH2) to form stable, irreversible amide bonds.


Biotin-TFP

Biotin-TFP esters have similar applications as the PFP esters, but are more stable in aqueous solution. It reacts with primary amino (-NH2) to form stable, irreversible amide bonds.


Fmoc-Lys (biotin-PEG)-OH

These reagents are Lysine embedded biotin PEG derivatives containing a carboxylic group and Fmoc-protected amine. Reaction of carboxylic with primary amino (-NH2) forms stable, irreversible amide bonds. The Fmoc group can be deprotected under basic condition to obtain the free amine which can be used for further conjugations.


Fmoc-N-amido-(PEG-biotin)-acid

This class of biotin PEG derivative contains a carboxylic group and Fmoc-protected amine. Reaction of carboxylic with primary amino (-NH2) forms stable, irreversible amide bonds. The Fmoc group can be deprotected under basic condition to obtain the free amine which can be used for further conjugations.


N-Boc-Biocytin

This class of biotin PEG derivative contains a carboxylic group and Boc-protected amine. Reaction of carboxylic with primary amino (-NH2) forms stable, irreversible amide bonds. The Boc group can be deprotected under acidic condition to obtain the free amine which can be used for further conjugations.


PEG-Biotin-PEG

3-arm PEGylated biotin PEG derivative that allows reaction with two amine-containing moiety to form stable, irreversible amide bonds.



Biotin-PEG-Oxyamine and Biotin Hydrazide are aldehyde-reactive biotinylation reagents that contain PEG spacer arms for increased hydrophilicity.
Carbonyl Reactive

Biotin-PEG-Oxyamine and Biotin Hydrazide are aldehyde-reactive biotinylation reagents that contain PEG spacer arms for increased hydrophilicity.

Biotin Hydrazide

Hydrazine moiety reacts with an aldehyde to form semi-permanent hydrazone bonds.


Biotin-PEG-Oxyamine

Aminooxy reacts with an aldehyde to form an oxime bond. If a reductant is used, it will form a hydroxylamine linkage.



A class of biotin compounds containing a PEG spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. PEGylation increases water solubility to the biotinylated molecule.
Carboxyl Reactive

A class of biotin compounds containing a PEG spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. PEGylation increases water solubility to the biotinylated molecule.

Biotin Amine

This is a class of biotinylated reagents containing an amino group which can efficiently react with acids, succinimidyl-active esters, or pentafluorophenyl esters. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.



Cleavable biotinylation reagents for selective enrichment of azide, alkyne, NHS active ester, oxyamine, … containing biomolecules.
Cleavable Biotin

Cleavable biotinylation reagents for selective enrichment of azide, alkyne, NHS active ester, oxyamine, … containing biomolecules.

Disulfide Biotin

A class of biotinylated reagents with an SS-bond or a disulfide bridge. These reagents can be cleaved by the reduction of disulfide bonds using reducing agents such as DTT, BME and TCEP to yield free sulfhydryls.


Dde Biotin

A class of biotin probes with Dde protecting group that allows the efficient release of captured biotinylated molecules from streptavidin under mild conditions with hydrazine.


Diazo Biotin

A class of biotin probes with diazo group that allows the efficient release of captured biotinylated molecules from streptavidin using sodium dithionite (Na2S2O4).


Diol Biotin

A class of biotin probes with diol group that allows the efficient release of captured biotinylated molecules from streptavidin using sodium periodate (NaIO4).


Photocleavable Biotin

A class of photoclevable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin using near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).


UV Cleavable Biotin



Azido-, alkyne-, and DBCO containing biotinylation reagents for bioconjugation, biolabeling and chemical modification. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.
Click Chemistry

Azido-, alkyne-, and DBCO containing biotinylation reagents for bioconjugation, biolabeling and chemical modification. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Biotin Alkyne

This is a class of biotinylated reagents containing an alkyne group which can efficiently react with azide using Cu(I)-catalyzed Click Chemistry. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.


Biotin Azide

This is a class of biotinylated reagents containing an azide group which can efficiently react with alkyne using Cu(I)-catalyzed Click Chemistry or react with DBCO using copper-free Click Chemistry. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.


Biotin DBCO

This is a class of biotinylated reagents containing an alkyne group which can efficiently react with azide using copper-free Click Chemistry. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.



Desthiobiotin is a single-ring, sulfur-free analog of biotin that provides soft-release elution from streptavidin.
Desthiobiotin

Desthiobiotin is a single-ring, sulfur-free analog of biotin that provides soft-release elution from streptavidin.

Desthiobiotin

Desthiobiotin is a single-ring, sulfur-free analog of biotin that provides soft-release elution from streptavidin.



A class of biotin labeling reagents such as Biotin-PEG-Mal and Biotin-HPDP used to specifically biotinylate antibodies and other biomolecules at sulfhydryl groups.
Sulfhydryl Reactive

A class of biotin labeling reagents such as Biotin-PEG-Mal and Biotin-HPDP used to specifically biotinylate antibodies and other biomolecules at sulfhydryl groups.

Biotin-Mal

This is a class of biotinylated reagents containing a maleimide group which is widely used for labeling of proteins and other thiolated biomolecules. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.


Biotin-HPDP

This is a class of biotinylated reagents containing a HPDP group which can react with sulfhydryls in nearly neutral buffers to form reversible disulfide bonds.



Methyltetrazine-activated biotinylation reagents react with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond.
Tetrazine Ligation

Methyltetrazine-activated biotinylation reagents react with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond.

Tetrazine-PEG-Biotin

Methyltetrazine-activated biotinylation reagents react with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.



Biotin labeling regents that contain hydroxyl group, halogen (ie. Iodide)
Other Labeling Reagents

Biotin labeling regents that contain hydroxyl group, halogen (ie. Iodide)

Biotin Alcohol

This is a class of biotinylated reagents containing a hydroxyl group which enables further derivatization or replacement with other reactive functional groups. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.


Miscellaneous Biotin Reagents