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MagicLink PE Antibody Conjugation Kits
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Protein Crosslinking Kit
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MagicLink PE Antibody Conjugation Kits (1x50ug)
MagicLink PE Antibody Conjugation Kits
Catalog #: BP-50094
Catalog #
Unit
Price
Qty
BP-50093
3x10ug
$275.00
BP-50094
1x50ug
$280.00
BP-50095
3x50ug
$450.00
BP-50096
1x100ug
$400.00
BP-50097
3x100ug
$500.00
BP-50098
1x500ug
$600.00
BP-50099
3x500ug
$1800.00
Usually ships within 24 hours.
Would you like to inquire about custom quantity?
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Overview
R-Phycoerythrin (PE, 250K), a fluorescent protein from the phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.
MagicLink™ is based on instant reaction between functional groups MAGIC and LINK. By following the easy protocol provided in the kits, the end users can activate their antibody with MAGIC NHS to get MAGIC-antibody. It is then instantly reacted with LINK pre-activated PE (provided in the kits) to achieve PE-antibody conjugates.
These kits are specifically formatted and optimized to conjugate 10µg, 50µg, 100µg, and 500µg of antibodies.
Novelty: 3rd generation protein crosslink technology. No DTT, TCEP, catalyst, quencher, or ligands needed.
Yield: conjugation efficiency around 95-100%; no antibody loss.
Reaction Rate: 10 seconds hands on time; real instant reaction; the whole process takes less than 1 hour!
Dye Stability: specially engineered to maintain its stability in the process and storage; dye degradation for PE and its tandem form will cause the spillover;
Flexible Size: select your own antibody amount size, 10µg, 50µg, 100µg, or 500µg.
Linkage Stability: most stable covalent bond conjugates on the market; much better than thiol-maleimide chemistry!
Consistence: good reproducibility from batch to batch.
Antibody Selection: any protein/antibody, or antibody fragment.
Purification: not need for post-conjugation.
Components
A. MAGIC NHS (MW ~900)
B. LINK activated APC
C. Reaction Buffer
D. Storage Buffer
Application
Flow cytometry, fluorescence-based detection, IHC application, western blot, ELISA, microarray assays, and other applications that require high sensitivity.
Images
Protocol
BP50093 BP50099
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