Azido-PEG4-Glu-Gly-Cit-PAB-MMAE possesses a cathepsin-cleavable Glu-Gly-Cit (EGCit) tripeptide, an azido-PEG4 spacer, a PABC linker, and a MMAE payload. Azido enables click chemistry with alkyne-bearing moieties. The peptide exhibits remarkable stability in the blood, resisting degradation and immune attacks, while enabling subtle and untraceable payload release within cells.
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