N4-Bz-3'-O-(4,4'-dimethoxy trityl)-2'-O-(2-MOE)-5-methylcytidine-5'-CED phosphoramidite, including a 2'-O-MOE group, can be integrated into oligonucleotide phosphoramidites for making antisense RNA. The 2'-MOE backbone improves duplex stability, nuclease resistance, and has low toxicity.
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