Desthiobiotin-NHS ester is a small molecule with a desthiobiotin and an NHS ester. Desthiobiotin is a sulfur-free analogue of biotin which binds streptavidin with slightly less strength than biotin, which provides it with a soft-release characteristic that is useful for in pull-down assays by minimizing co-elution with endogenous biotinylated molecules. The NHS ester easily reacts with amines to form amides.
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