N-(Methyltetrazine-PEG4)-N-Biotin-PEG4-NHS is a branched biotinylation tool that enables copper-free click chemistry with an NHS ester group and a TCO reactive reagent. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is the fastest bioorthogonal reaction with exceptional selectivity. The NHS group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. Reagent grade, for research use only.
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