Thiazole Orange (TO) NHS Ester is an asymmetric cyanine dye whose fluorescence highly depends on the local environment. It's fluorescence increases a thousandfold when ?? is introduced into double-stranded DNA and RNA. The NHS esters can react specifically and efficiently with primary amines such as the side chain of lysine residue or aminosilane-coated surfaces at neutral or slight basic conditions to form a covalent bond. Reagent grade, for research use only.
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