N-(NHS ester-PEG4)-N-bis(PEG4-azide) functions as a branched linker containing two azides and a terminal NHS ester. The azide is reactive towards strained cyclooctynes and other alkynes using click chemistry. The NHS ester is able to react specifically and efficiently with amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces). PEG linkers provide high aqueous solubility, and altering PEG length can alter DMPK.
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