Cy3 DIC18 is an orange-red fluorescent carbocyanine dye widely utilized as a lipophilic tracer. Its long hydrocarbon chains integrate into cell membranes, rendering it weakly fluorescent until incorporation. DiI diffuses laterally to stain entire cells, making it effective for anterograde and retrograde neuronal tracing. In intact tissue, minimal transfer occurs between labeled and unlabeled cells, though slight transfer may happen post-membrane disruption, such as during sectioning.
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