DiO, lipophilic tracer is a green fluorescent and lipophilic carbocyanine dye. It is commonly used as an anterograde and retrograde neuronal tracer in both living and fixed tissues and cells. DiO uniformly labels neurons through diffusion in the plasma membrane, and while it does not transfer from labeled to unlabeled cells in intact tissue, some transfer may occur after membrane disruption, such as during sectioning.
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