AF 488 tyramide is a water-soluble green fluorescent dye used in tyramide signal amplification (TSA) for enhancing fluorescence in immunohistochemistry, immunocytochemistry, and fluorescence in situ hybridization. TSA relies on horseradish peroxidase (HRP) to convert tyramine-containing substrates into highly reactive radicals that covalently bind to nearby proteins. AF 488 tyramide can be used with any HRP-conjugated molecules to stain cells and tissues in immunofluorescence assays.
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