Sulfo-Cy5.5 tyramide is integral to tyramide signal amplification (TSA), enhancing fluorescent signals in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). Utilizing horseradish peroxidase (HRP), it converts tyramine-containing substrates into reactive radicals. This enables the detection of low-concentration targets and reduces background staining without compromising signal intensity. Sulfo-Cy5.5 tyramide can be used with HRP-conjugated molecules, such as antibodies or streptavidin, for immunofluorescence staining of cells and tissues.
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