Sulfo-Cy7 tyramide is crucial for enhancing fluorescent signals via tyramide signal amplification (TSA) in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). Through horseradish peroxidase (HRP), it converts tyramine-containing substrates into reactive radicals. This enables the detection of low-concentration targets and reduces background staining without compromising signal intensity. Sulfo-Cy7 tyramide can be used with HRP-conjugated molecules, such as antibodies or streptavidin, for immunofluorescence staining of cells and tissues.
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