Ac4ManNAl is a tetraacetylated N-(4-pentynoyl)-mannosamine, is an alkyne-labeled monosaccharide used for studying glycoproteins. It's cell-permeable and replaces the natural monosaccharide ManNAc intracellularly. This metabolic labeling tool allows specific detection of glycoproteins. Detection is achieved via Cu(I)-catalyzed click reaction (CuAAC) with fluorescent-labeled azides or biotin-azide, providing insight into glycoprotein dynamics in vivo.
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