Methyltetrazine-amido-N-bis(PEG4-DBCO) represents a PEG derivative that includes a methyltetrazine group and two DBCO groups. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is the fastest bioorthogonal reaction with exceptional selectivity. DBCO is a click chemistry handle that is frequently used to conjugate with azides on target molecules. The PEG4 chains increase the water solubility of the compound.
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