Photoclick sphingosine is a photoreactive probe for the study of sphingosine metabolism. It contains a terminal clickable alkyne moiety and a photoactivatable diazirine group that allow its interaction with sphingosine binding proteins and photo-activated cross-linking of its interacting partners, respectively. Photoclick sphingosine has been used to monitor the metabolism of sphingosine and visualize protein-lipid interaction complexes in S1PL-/- mouse embryonic fibroblasts (MEFs).
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