PE-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 780 nm. PE-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, preactivated PE-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
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