Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE-Cy5 antibody (4µl reagent / 100µl of peripheral whole blood).
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These three dyes, BP Fluor 647, Cyanine 650, and Cy5, are bright green-fluorescent dye optimal for use with the 633, 650 nm argon laser. The three dyes have the same fluorophore backbone structure which contributes to the almost identical absorption and emission spectrum. They are only different from the number of soluble groups SO3Na, i.e. Cy5 has two SO3Na groups, and Cyanine 650 has three while BP Fluor 647 has four.
All these dyes are water soluble and pH-insensitive from pH 4 to pH 10. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution. These dyes are used for protein and antibody labeling, or nucleic acid applications with high labeling density for numerous bio applications.
All three dyes can be conjugated to primary antibody, secondary antibody, or other biomolecules for the applications below.