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Allophycocyanin (APC, 105K) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594, 633 nm, 640nm with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is chemically crosslinked to improve its stability for the fluorescent detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
MagicLink™ is based on instant reaction between functional groups MAGIC and LINK. By following the easy protocol provided in the kits, the end users can activate their antibody with MAGIC NHS to get MAGIC-antibody. It is then instantly to react with LINK pre-activated APC (provided in the kits) to achieve APC-antibody conjugates.
These kits are specifically formatted and optimized to conjugate 10µg, 50µg, 100µg, and 500µg of antibodies.
A. MAGIC NHS (MW ~900)
B. LINK activate APC
C. Reaction Buffer
D. Storage Buffer
Flow cytometry, fluorescence-based detection, IHC application, western blot, ELISA, microarray assays, and other applications that require high sensitivity.