N-(NHS ester-PEG3)-N-bis(PEG3-Mal) is a 3-arm PEG reagent with an NHS ester group and two maleimide moieties that are reactive toward thiols. Maleimide groups react specifically with sulfhydryl groups to form a stable thioether linkage when the pH is between 6.5 and 7.5. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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