Brassicasterol, a 28-carbon sterol, is produced by various unicellular algae (phytoplankton) and certain terrestrial plants. Its tail region contains a C-24 methyl analog with one double bond, providing low rigidity. As a C-24 alkyl derivative, its tail and body variations impact the size, mRNA encapsulation efficiency (EE), and transfection efficiency of lipid nanoparticles (LNPs). These structural features influence its potential in gene transfection capabilities.
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