Ergosterol, a sterol found in fungal cell membranes, plays a role in maintaining membrane integrity, akin to cholesterol in mammals. It features a moderately rigid C-24 methyl analog with one double bond in the tail region and another in the body region. As a C-24 alkyl derivative, its tail and body variations affect the size, mRNA encapsulation efficiency (EE), and transfection efficiency of lipid nanoparticles (LNPs).
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