Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines. BP Fluor 568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm. BP Fluor 568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.
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