TCO-PEG2-NHS ester serves as a heterobifunctional PEG linker comprised of a TCO click chemistry handle that readily reacts with tetrazine groups and NHS ester that directly and efficiently reacts with amine-bearing molecules. The PEG spacer imparts water solubility. The half-life of TCO is short as it will naturally isomerize into CCO (cis-cycloctene) and lose the reactivity. TCO compound is not recommended for long term storage.
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