Methyltetrazine-PEG4-PFP ester operates as a heterobifunctional PEG linker including methyltetrazine, a click chemistry handle used to efficiently react with trans-cyclooctenes (TCO), and PFP ester, an amine-reactive ester that is less susceptible toward hydrolysis compared to NHS ester. The hydrophilicity of the molecule is elevated via the PEG4 spacer.
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