Neuro-DiO is a green fluorescent, lipophilic carbocyanine dye optimized for neuronal tracing, with an excitation maximum at 497 nm and an emission maximum at 514 nm. It shares similarities with DiO, a commonly used tracer, but offers improved solubility in membranes and prevents nonfluorescent aggregate formation. Neuro-DiO labels neurons uniformly through membrane diffusion, with minimal transfer between labeled and unlabeled cells in intact tissue.
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