Di-4-ANEPPS is a voltage-sensitive dye from the Amino-Naphthyl-Ethenyl-Pyridinium (ANEP) family. Initially non-fluorescent, it becomes active when bound to cell membranes and responds to electrical potential changes. This fast-acting dye detects transient (millisecond) potential fluctuations in excitable cells like neurons, cardiac cells, and intact brains. Due to rapid cell internalization, it’s ideal for short-term studies.Di-4-ANEPPS can also serve as a plasma membrane and endocytosis marker.
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