RH 237 is a lipophilic cationic dye used as a fluorescent probe in biological research. It belongs to the rhodamine class of compounds, known for their high brightness and photostability in imaging applications. RH 237 primarily enables functional imaging of neurons, including membrane potential, synaptic activity, and ion channel function. In methanol, RH 237 exhibits an excitation peak at 550 nm and an emission peak at 786 nm. Notably, in cell membranes, the dye’s spectra experience blue shifts of approximately 20 nm for excitation and 80 nm for emission.
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