5-Vinyl-2’-deoxyuridine(VdU) is a synthetic thymidine analog used to study DNA synthesis and cell proliferation. It incorporates into DNA during the S-phase, replacing natural thymidine. VdU-labeled DNA can be detected with biotin or fluorescent dyes using a copper-free alkene-tetrazine reaction (IEDDA). This makes it a potential alternative to BrdU and EdU for DNA purification and cell imaging.
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