Bis-ANS is a high-affinity fluorescent probe for detecting nonpolar protein cavities, with hydrophobic rings that interact noncovalently with proteins. Nonfluorescent in water, bis-ANS fluoresces intensely in nonpolar environments, blue-shifting upon protein binding (excitation/emission: 390/523 nm). It’s ideal for monitoring protein folding, aggregation, and Aβ fiber detection.
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