FCCP (trifluoromethoxy carbonylcyanide phenylhydrazone), ATPase inhibitor is a potent mitochondrial uncoupling agent and H? ionophore. It dissipates mitochondrial membrane potential, uncoupling oxidative phosphorylation from ATP synthesis. FCCP is widely used to analyze mitochondrial function and induce mitophagy by disrupting membrane potential. Effects depend on concentration, with low doses preserving mitochondria and high doses promoting degradation.
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