Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue by intracellular esterases. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein Blue AM is a blue-emitting variant of the green fluorophore Calcein AM, which has been used to evaluate cell viability and cytotoxicity.
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