Name: Cy5 NHS Antibody Labeling Kit (1 x 100 ug)
Brand: BroadPharm
SKU: BP-50026
Price: 250.00 USD
Availability: InStock

Cy5 NHS Antibody Labeling Kit

Catalog #: bp-50026

Flow cytometry surface staining pattern of human peripheral whole blood stained with anti-human CD45 (NULLD1) PE-Cy™5 antibody.

Key Features and Details

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE-Cy™5 antibody (4 µl reagent / 100 µl of peripheral whole blood).

Overview

These three dyes, BP Fluor 647, Cyanine 650, and Cy5, are bright green-fluorescent dye optimal for use with the 633, 650 nm argon laser. The three dyes have the same fluorophore backbone structure which contributes to the almost identical absorption and emission spectrum. They are only different from the number of soluble groups SO3Na, i.e. Cy5 has two SO3Na groups, and Cyanine 650 has three while BP Fluor 647 has four.
All these dyes are water soluble and pH-insensitive from pH 4 to pH 10. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution. These dyes are used for protein and antibody labeling, or nucleic acid applications with high labeling density for numerous bio applications.
Components
1. active Dye, 1
2. Buffer, 1
3. Desalt column, 1
4. DMSO, 1 rxn 1 mL, 1
5. NaN3 3%, 0.5ml, 1
Application
All three dyes can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.
- Flow cytometric analysis of antibody surface-stained cells (FC)
- Flow cytometric analysis of intracellularly-stained cells ( ICFC)
- Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded. (IHC-F)
- IHC-P
- Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies. (ICC)
- Immunofluorescence Microscopy, Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides, or tissue preparations (IF)
- Immunodetection applied to formalin-fixed tissue sections of at least 300 microns in thickness, which are optically cleared by tissue clearing reagent (3-DIHC)
- IHC
- WB
Images

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) PE-Cy™5 antibody (4 µl reagent / 100 µl of peripheral whole blood).
Protocol

BP Fluor647 Cy5 Cyanie650 General protocol dye MAL labeling kit General protocol dye NHS labeling kit

Cy5 NHS Antibody Labeling Kit

Cy5 NHS Antibody Labeling Kit

Key Features and Details

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Overview

Components

Application

Images

Protocol

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