HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 μg/ml Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (red) in 5% FBS for two hours at room temperature, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD64 (NULL.1) Alexa Fluor® 647 antibody prepared by MagicLink™ Alexa Fluor 647 Mal kit, 4 µl reagent / 100 µl of peripheral whole blood).