Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (ICRF44) Alexa Fluor® 488 antibody prepared by MagicLink Alexa Fluor 488 Mal kit (Cat. # BP-50036, 4 µl reagent / 100 µl of peripheral whole blood).
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BP Fluor 488 is a green fluorescent dye for the 488 nm argon laser line. BP Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission spectra comparable to Fluorescein (FITC), but are much brighter, far more photostable and less sensitive to pH changes between pH 4 and 10. BP Fluor 488 conjugates are compatible with common fluorescein equipment, settings, or filters and are ideally suitable for all applications in fluorescence microscopy and flow cytometry with high requirements in sensitivity. BP Fluor 488 shows more persistent and brighter fluorescence than Fluorescein in epifluorescence microscopes even without the addition of anti-fading agents to aqueous mounting media. In permanent organic mounting media BP Fluor 488 is also more fluorescent and long-term stable than FITC.
The features of BP Fluor 488 are the multiple negative charges which can significantly reduce conjugates aggregation in the bio application.
BP Fluor 488 can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.