Name: Cy3 Mal Antibody Labeling Kit (3 x 100 ug)
Brand: BroadPharm
SKU: BP-50040
Price: 140.00 USD
Availability: InStock

Cy3 Mal Antibody Labeling Kit

Catalog #: bp-50040

Graphic shows CWR22R cells is fixed with 4% paraformaldehyde for 20 min, blocked by 3% BSA, and cultured at 4°C overnight.

Key Features and Details

CWR22R cells is fixed with 4 % paraformaldehyde for 20 min, blocked by 3 % BSA, and cultured at 4 °C overnight with Anti PSMA monoclonal antibodies (NULL : 100), and incubated with Cy3- anti mouse IgG antibody at room temperature for 2 h. DAPI nucleic stain was performed in blue.

Overview

Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.
The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (>12 carbons) onto the core fluorophore.
Components
1. active Dye, 3
2. Buffer, 1
3. Desalt column, 3
4. DMSO, 1 rxn 1 mL, 1
5. NaN3 3%, 0.5 mL, 1
Application

The dye is used to conjugated to primary antibody, secondary antibody.
The applications include immunolabeling nucleic acid labeling, fluorescence microscopy, and flow cytometry.
Images

CWR22R cells is fixed with 4 % paraformaldehyde for 20 min, blocked by 3 % BSA, and cultured at 4 °C overnight with Anti PSMA monoclonal antibodies (NULL : 100), and incubated with Cy3- anti mouse IgG antibody at room temperature for 2 h. DAPI nucleic stain was performed in blue.
Protocol

CY3 General protocol dye MAL labeling kit General protocol dye NHS labeling kit

Cy3 Mal Antibody Labeling Kit

Cy3 Mal Antibody Labeling Kit

Key Features and Details

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Overview

Components

Application

Images

Protocol

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