Name: 800CW Mal Antibody Labeling Kit (3 x 100 ug)
Brand: BroadPharm
SKU: BP-50052
Price: 450.00 USD
Availability: InStock

800CW Mal Antibody Labeling Kit

Catalog #: bp-50052

Diagram showing 800CW probe being used as fluorescent probes for intraoperative navagation

Key Features and Details

800CW probe is used as fluorescent probes for intraoperative navagation

Overview

800CW (IRDye 800CW acid equivalent) is a near-infrared fluorescent dyes. The unique structure has 4 sulfonates which make it as a high water soluble and less aggregation in aqueous solution. 800CW is used for protein and antibody labeling, or nucleic acid applications with high labeling density.
800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye based on its enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.
Components
1. active Dye, 3
2. Buffer, 1
3. Desalt column, 3
4. DMSO, 1 rxn 1 mL, 1
5. NaN3 3%, 0.5 mL, 1
Application
800CW dye can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below.
- Western blot
- flow cytometry
- immunocytochemical assays, including In-Cell Western assays and On-Cell
- Western cell-based assays
- protein arrays
- microscopy
- tissue section imaging
- in vivo imaging and optical probe development
Images

800CW probe is used as fluorescent probes for intraoperative navagation
Protocol

800CW General protocol dye MAL labeling kit General protocol dye NHS labeling kit
Reference
1. Marshall, M.V., Draney, D., Sevick-Muraca, E.M. et al. Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats. Mol Imaging Biol 12, 583 594 (2010).
2. Kijanka, M., Warnders, FJ., El Khattabi, M. et al. Rapid optical imaging of human breast tumour xenografts using anti-HER2 VHHs site-directly conjugated to IRDye 800CW for image-guided surgery. Eur J Nucl Med Mol Imaging 40, 1718 1729 (2013).
3. Ruimin Huang, Jelena Vider, Joy L. Kovar, D. Michael Olive, Ingo K. Mellinghoff, Philipp Mayer-Kuckuk, Moritz F. Kircher and Ronald G. Blasberg;Integrin ?v?3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma ;Clin Cancer Res October 15 2012 (18) (20) 5731-5740;
4. Linder, Karen E., et al. "Synthesis, in vitro evaluation, and in vivo metabolism of fluor/quencher compounds containing IRDye 800CW and Black Hole Quencher-3 (BHQ-3)." Bioconjugate chemistry 22.7 (2011): 1287-1297.
5. Bujie Du, Xingya Jiang, Yingyu Huang, Siqing Li, Jason C Lin, Mengxiao Yu, and Jie Zheng
  Tailoring Kidney Transport of Organic Dyes with Low-Molecular-Weight PEGylation
  Bioconjugate Chemistry 2020 31 (2), 241-247
6. Francisco Romero Pastrana, John M. Thompson, Marjolein Heuker, Hedzer Hoekstra, Carly A. Dillen, Roger V. Ortines, Alyssa G. Ashbaugh, Julie E. Pickett, Matthijs D. Linssen, Nicholas M. Bernthal, Kevin P. Francis, Girbe Buist, Marleen van Oosten, Gooitzen M. van Dam, Daniel L. J. Thorek, Lloyd S. Miller & Jan Maarten van Dijl (2018) Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probe, Virulence, 9:1, 262-272,

800CW Mal Antibody Labeling Kit

800CW Mal Antibody Labeling Kit

Key Features and Details

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Overview

Components

Application

Images

Protocol

Reference

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