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MagicLink HRP Antibody Conjugation Kit
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Products
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Protein Crosslinking Kit
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MagicLink HRP Antibody Conjugation Kit (5 x 1 mg)
MagicLink HRP Antibody Conjugation Kit
Catalog #: bp-50062
Catalog #
Unit
Price
Qty
BP-50002
1 x 100 ug
$160.00
BP-50001
3 x 100 ug
$335.00
BP-50000
1 x 1 mg
$490.00
BP-50061
1 x 5 mg
$1800.00
BP-50062
5 x 1 mg
$1850.00
Usually ships within 24 hours.
Would you like to inquire about custom quantity?
Inquire
Overview
HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue.
BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™ chemistry and kit do not need TCEP, DTT, or catalysts at all!
First, antibody reacts with MAGIC NHS ester to yield the MAGIC activated antibody which instantly reacts with LINK preactivated HRP to yield antibody-HRP conjugate with a stable ring linkage.
Novelty:
3rd generation protein crosslink technology
Yield:
conjugation efficiency around 95-100%
Rate:
instant reaction; the whole process less than 1 hour
Linkage stability:
most stable covalent bonded conjugates on the market
Consistence:
good reproducibility from batch to batch
Flexible:
use your own HRP and antibody at your desired ratio
Shelf life:
long, stable freeze dried ship at ambient temperature
Antibody selection:
any proteins/antibody, antibody fragment
Post-conjugation clean-up step not necessary
HRP activity:
> 250 U/mg
Application
MagicLink HRP antibody Labeling Kits are used to conjugate HRP with primary antibody, secondary antibodies, other biomolecules for the bioassay below.
Western blotting (WB)
Immunohistochemistry - Paraffin
ELISA
DotBlot
ELISA Capture
ELISA Detection
ELISPOT
Floe cytometry
Immunohistochemistry
Immunohistochemistry - Frozen
Immunoprecipitation
Images
Protocol
MagicLink™ HRP Antibody Labeling Kits
Reference
Tougard C, Tixier-Vidal A, Avrameas S.; Comparison between peroxidase-conjugated antigen or antibody and peroxidase-anti-peroxidase complex in a postembedding procedure; J Histochem Cytochem (1979): 1630
Sternberger LA, Petrali JP.; The unlabeled antibody enzyme method. Attempted use of peroxidase-conjugated antigen as the third layer in the technique; J Histochem Cytochem (1977): 1036
Strakova Z, Barancik M, Lukacova D, Angyal R, Slosarcikova L, Horakova K.; Peroxidase labelled monoclonal antibody against light chains of human cardiac myosin; Gen Physiol Biophys (1991): 63
Tijssen P, Kurstak E.; Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassays; Anal Biochem (1984): 451
Boorsma DM, Cuello AC, van Leeuwen FW.; Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance; J Histochem Cytochem (1982): 1211
Broorsma DM, Steefkerk JG, Kors N.; Peroxidase and fluorescein isothiocyanate as antibody markers. A quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate; J Histochem Cytochem (1976): 1017
Nakane PK, Kawaoi A.; Peroxidase-labeled antibody. A new method of conjugation; J Histochem Cytochem (1974): 1084
Tresca JP, Ricoux R, Pontet M, Engler R.; Comparative activity of peroxidase-antibody conjugates with periodate and glutaraldehyde coupling according to an enzyme immunoassay; Ann Biol Clin (Paris) (1995): 227
Presentini R, Terrana B.; Influence of the antibody-peroxidase coupling methods on the conjugate stability and on the methodologies for the preservation of the activity in time; J Immunoassay (1995): 310
Other Related Products
MagicLink™ HRP Antibody Conjugation Kit (1 x 100 ug)
MagicLink™ HRP Antibody Conjugation Kit (3 x 100 ug)
MagicLink™ HRP Antibody Conjugation Kit (1 x 1 mg)
MagicLink HRP Antibody Conjugation Kit (1 x 5 mg)