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R-Phycoerythrin (PE, 250K), a fluorescent protein from the phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.
MagicLink™ is based on instant reaction between functional groups MAGIC and LINK. By following the easy protocol provided in the kits, the end users can activate their antibody with MAGIC NHS to get MAGIC-antibody. It is then instantly reacted with LINK pre-activated PE (provided in the kits) to achieve PE-antibody conjugates.
These kits are specifically formatted and optimized to conjugate 10µg, 50µg, 100µg, and 500µg of antibodies.
A. MAGIC NHS (MW ~900)
B. LINK activated PE
C. Reaction Buffer
D. Storage Buffer
Flow cytometry, fluorescence-based detection, IHC application, western blot, ELISA, microarray assays, and other applications that require high sensitivity.