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MagicLink™ Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc.
This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get the conjugation in less than 2 hours.
These kits are specifically optimized to label antibodies at scales of 100 ug and 1 mg. The kits are packaged as convenient single-use microtubes, eliminating difficulties associated with weighing small quantities of reagent.