This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as click chemistry, in the presence of a copper (Cu)-containing catalyst. E-Cholesterol Alkyne (19-ethynylcholesterol) represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.
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