N-(Mal-PEG8-carbonyl)-N-bis(PEG8-NHS ester) is a trifunctional linker containing two activated NHS esters and a maleimide group. The NHS esters are strongly reactive towards forming amides and esters while the maleimide is a thiol-specific covalent ligand that is most often used to label cysteine residuess along protein chains. The use of a tertiary amide as the junction combining these allows for hydrogen bonding and increases this compound’s aqueous solubility.
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