RAPID DiO is a cyanine dye with green fluorescence—an unsaturated analog of DiO. It labels cell membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. Initially weakly fluorescent, it becomes more so upon incorporation into membranes. RAPID DiO diffuses laterally, staining the entire cell, and serves as an anterograde and retrograde tracer of neurons. Notably, it exhibits about 50% faster migration kinetics than DiO.
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