Fluo-4 AM is a cell-permeable calcium indicator that becomes highly fluorescent upon Ca²? binding (excitation/emission at 494/506 nm). It is metabolized by intracellular esterases, enabling effective visualization and measurement of intracellular Ca²?. This dye is widely used in applications such as microscopy, flow cytometry, spectrofluorometry, and high-throughput microplate assays.
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