S-Acetylmercaptosuccinic anhydride (SAMSA) is an amine-reactive reagent that introduces sulfhydryl groups through nucleophilic attack by amines, resulting in amide bond formation. During this ring-opening reaction, a free carboxylate group is generated, imparting a negative charge to the molecule. This charge reversal can influence protein activity and molecular conformation, making the reagent useful for controlled protein modification and bioconjugation studies.
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